15 - 18 Oct. 2024

Plaza Verde at Numazu, Shizuoka, Japan


Programme

Day 1 (15 Oct. 2024)

09:15 - 10:35  Registration
10:35 - 10:45  Welcome address
10:45 - 12:30
Session1: Bacterial SMC
Chair: Eugene Kim
10:45 - 11:10 (I-1) Replisomes are barriers to SMC-complex-mediated DNA-loop extrusion
Xindan Wang - Indiana University, USA
11:10 - 11:35 (I-2) Probing mechanisms of DNA loop extrusion using plasmid restriction by SMC JET nuclease
Stephan Gruber - University of Lausanne, Switzerland
11:35 - 12:00 (I-3) Structural basis for DNA capture by the MukBEF SMC complex and its disruption by a viral inhibitor
Frank Bürmann - University of Oxford, UK
12:00 - 12:15 (S-1) MukBEF ensures proper chromosome segregation of highly transcribed regions
Andres Canela - Kyoto University, Japan
12:15 - 12:30 (S-2) New insights into bacterial condensin activities
Virginia Lioy - I2BC, France
   
12:30 - 13:30  Lunch
   
13:30 - 15:15
Session2: SMC Mechanism
Chair: Jan-Michael Peters
13:30 - 13:55 (I-4) Molecular determinants of loop extrusion by single cohesin molecules
Maxim Molodtsov - The Francis Crick Institute, UK
13:55 - 14:20 (I-5) Single-molecule biophysics studies of DNA loop extrusion by SMC motor proteins
Cees Dekker - Delft University of Technology, The Netherlands
14:20 - 14:45 (I-6) Biochemical and Biophysical Studies of the Caenorhabditis elegans Dosage Compensation Complex
Christian Haering - Julius Maximilian University of Wuerzburg, Germany
14:45 - 15:00 (S-3) New twists of loop extrusion
Leonid Mirny – MIT, USA
15:00 - 15:15 (S-4) Molecular simulations reveal that a prokaryotic SMC with an asymmetric kleisin path repositions unidirectionally on DNA
Masataka Yamauchi - Kyoto University, Japan
   
15:15 - 15:45  Break
   
15:45 - 17:30
Session3: Chromosome organization 1
Chair: Sevinc Ercan
15:45 - 16:10 (I-7) Functional interplay of condensin I and topoisomerase IIa on single DNA compaction
Tomoko Nishiyama - Kyoto University, Japan
16:10 - 16:35 (I-8) Life without Loop Extrusion?
Frank Uhlmann - The Francis Crick Institute, UK
16:35 - 17:00 (I-9) PolII-based chromosome folding regulation in yeast
Romain Koszul - Institut Pasteur / CNRS, France
17:00 - 17:15 (S-5) Eukaryotic-like mechanism of chromosomal domain formation by archaeal SMC, a roadblock NAP, and DNA structure
Naomichi Takemata - Kyoto University, Japan
17:15 - 17:30 (S-6) How does Wapl restrict cohesin-dependent DNA looping?
Henri Mboumba – CBI, CNRS, France
   
18:00 – 20:00  Welcome Reception
   

Day 2 (16 Oct. 2024)

09:00 - 10:45
Session4: Chromosome Organization 2
Chair: Camilla Björkegren
09:00 - 09:25 (I-10) Genome control by SMC ligands
Benjamin Rowland - The Netherlands Cancer Institute, The Netherlands
09:25 - 09:50 (I-11) Assembly and interactions of condensin and other scaffold proteins during mitotic chromosome formation
William Earnshaw - University of Edinburgh, UK
09:50 - 10:15 (I-12) Single-nucleosome imaging unveils that condensins and nucleosome–nucleosome interactions differentially constrain chromatin to organize mitotic chromosomes
Kazuhiro Maeshima - National Institute of Genetics, Japan
10:15 - 10:30 (S-7) High levels of Condensin I promote greater chromatin compaction during mitosis in pluripotent stem cells
Raquel A. Oliveira - Católica Biomedical Research Centre / Instituto Gulbenkian de Ciência, Portugal
10:30 - 10:45 (S-8) Molecular Mechanism Targeting Condensin to Pericentromeres and rDNA
Adele Marston - University of Edinburgh, UK
   
10:45 - 11:15  Break
   
11:15 - 12:40
Session5: Smc5/6
Chair: Stephan Gruber
11:15 - 11:35 (I-13) The Smc5/6 complex, DNA supercoiling and topoisomerases
Camilla Björkegren - Karolinska Institute, Sweden
11:35 - 12:00 (I-14) Functional mechanisms of the Smc5/6 complex
Xiaolan Zhao - Memorial Sloan Kettering Cancer Center, USA
12:00 - 12:25 (I-15) Dimeric SMC complexes-mediated DNA loop extrusion: Insights from Wadjet and Smc5/6
Eugene Kim - Max Planck Institute of Biophysics, Germany
12:25 - 12:40 (S-9) Human SMC5/6 securely links two ssDNA molecules
Luis Aragon - MRC LMS, UK
   
12:40 - 15:30  Lunch
   
15:30 - 17:15
Session6: SMC and Diversity
Chair: Tatsuya Hirano
15:30 - 16:10 (I-16) Structural basis for ABCB1 multi-drug transporter mechanism
Hiroaki Kato - Kyoto University, Japan
16:10 - 16:35 (I-17) Eukaryotic SMC complexes through an evolutionary lens
Jolien van Hooff - Wageningen University & Research, The Netherlands
16:35 - 17:00 (I-18) The role of the SMC-related protein, coalescin, in archaeal chromosome organization
Stephen D Bell - The Ohio State University, USA
17:00 - 17:15 (S-10) ClsN, an Archaeal Sulfolobus SMC Protein, Induces Chromosome Compartmentalization via Bridging-Induced Phase Separation
Je-Kyung Ryu - Seoul National University, Korea
   
17:15 - 19:15  Poster Session 1
19:15 - 20:00  Extra Time
   

Day 3 (17 Oct. 2024)

09:00 - 10:45
Session7: Cohesin and Condensin 1
Chair: Tomoko Nishiyama
09:00 - 09:25 (I-19) Hybrid incompatibility in cohesin protection leads to egg aneuploidy and female sterility
Takashi Akera - NIH, USA
09:25 - 09:50 (I-20) The role of DNA catenation in Sister Chromatid Cohesion
Madhusudhan Srinivasan - University of Oxford, UK
09:50 - 10:15 (I-21) How cohesin folds the genome by loop extrusion
Jan-Michael Peters - IMP, Austria
10:15 - 10:30 (S-11) The cohesin ATPase cycle is mediated by specific conformational dynamics and interface plasticity of SMC1A and SMC3 ATPase domains
Marina Vitoria Gomes - University of Wuerzburg, Germany
10:30 - 10:45 (S-12) Synergistic Mechanisms of Topological Simplification by SMC and Topoisomerase
Cleis Battaglia - University of Edinburgh, UK
   
10:45 - 11:15  Break
   
11:15 - 12:40
Session8: Cohesin and Condensin 2
Chair: Benjamin Rowland
11:15 - 11:35 (I-22) Condensin collaborates with topoisomerases at replication forks to facilitate fork reversal in response to replication stress
Armelle Lengronne – Institute of Human Genetics, CNRS, France
11:35 - 12:00 (I-23) Supermolecular clusters that drive mitotic chromosome assembly
Toru Hirota - Japanese Foundation for Cancer Research, Japan
12:00 - 12:25 (I-24) Toward a deep understanding of mitotic chromosome assembly
Tatsuya Hirano – RIKEN, Japan
12:25 - 12:40 (S-13) Condensin is Diversely Regulated by Short Linear Motifs
Erin Cutts - University of Sheffield, UK
   
12:40 - 13:30  Lunch
   
13:30 - 15:15
Session9: SMC and Gene Regulation
Chair: Ana Losada
13:30 - 13:55 (I-25) Transcriptional Dysregulation in Cohesinopathy
Katsuhiko Shirahige - The University of Tokyo, Japan
13:55 - 14:20 (I-26) X-chromosome dosage compensation: a single molecule perspective on a condensin-driven gene regulatory process
Barbara Meyer - Howard Hughes Medical Institute and U. C. Berkeley, USA
14:20 - 14:45 (I-27) In vivo regulators of condensin binding and function
Sevinc Ercan - New York University, USA
14:45 - 15:00 (S-14) Repression of sub-telomeric gene expression by SMC complexes in budding yeast.
Shikha Laloraya - Indian Institute of Science, India
15:00 - 15:15 (S-15) Crossover repair recruits condensin to reorganize the meiotic chromosome axis
Victor Leon - New York University, USA
   
15:15 - 17:30  Poster Session 2
18:30 – 20:30  Dinner
   

Day 4 (18 Oct. 2024)

09:00 - 10:45
Session 10: SMC and Genome Stability
Chair: Xiaolan Zhao
09:00 - 09:25 (I-28) Searching for homology: RecN drives RecA action in homologous recombination
Anjana Badrinarayanan - National Centre for Biological Sciences, India
09:25 - 09:50 (I-29) Models of RecN function in bacterial homology search
Anton Goloborodko - IMBA, Austria
09:50 - 10:15 (I-30) A convergent evolutionary strategy for inactivation of MRN at telomeres
Antony Oliver - Genome Damage and Stability Centre, University of Sussex, UK
10:15 - 10:30 (S-16) Inhibiting SMC Complexes with Peptides: A Heads-Up
Itay Onn - Bar-Ilan University, Israel
10:30 - 10:45 (S-17) Combination of AID2 and BromoTag expands the utility of degron-based protein knockdowns
Masato Kanemaki - National Institute of Genetics, Japan
   
10:45 - 11:15  Break
   
11:15 - 12:25
Session 11: SMC and Disease
Chair: Frank Uhlmann
11:15 - 11:35 (I-31) Nuclear and cytoplasmic functions of DDX11, the Warsaw breakage syndrome DNA helicase
Francesca Pisani - Institute of Biochemistry and Cell Biology, CNR, Italy
11:35 - 12:00 (I-32) NIPBL haploinsufficiency accelerates neural development in Cornelia de Lange Syndrome-modelling human cortical brain organoids
Debbie L C van den Berg - Erasmus MC Rotterdam, The Netherlands
12:00 - 12:25 (I-33) NIPBL/Cohesin imbalance rewires enhancer-promoter contacts and contributes to poor prognosis in STAG2 mutant Ewing sarcoma
Ana Losada - Spanish National Cancer Research Centre, Spain
   
12:25 - 12:30  Conclusion Remarks
12:30 - 15:00  Lunch and Free Discussion
   

Poster List

P-1

Condensin-Driven Bipartite Organisation of Vertebrate Centromeres in Mitosis

    Kumiko Samejima1, Moonmoon Deb1, Adam Buckle2, Chris A. Brackley3, Daniel Robertson1, Tetsuya Hori4, Shaun Webb1, Tatsuo Fukagawa4, Nick Gilbert2, William C. Earnshaw1

  • 1 Wellcome Centre for Cell Biology, Institute of Cell Biology, University of Edinburgh, Edinburgh, UK
  • 2 MRC Human Genetics Unit, Institute of Genetics & Molecular Medicine, University of Edinburgh, Edinburgh, UK
  • 3 SUPA School of Physics and Astronomy, University of Edinburgh, Edinburgh, UK
  • 4 Laboratory of Chromosome Biology, Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka, Japan
    •

P-2

Structural maintenance of chromosomes (SMC) proteins and pathological phenotypes in human: Review literature and Insight form prototype disease Cornelia de Lange syndrome (CDLS)

    Prashant Kumar Verma1, and Nowneet Kumar Bhat2

  • 1 Department of pediatrics, Chairperson of Medical Genetic Unit, AIIMS Rishikesh, India
  • 2 Department of pediatrics, AIIMS Rishikesh, India
    •

P-3

SINE retrotransposons regulate higher order genome functions by recruiting H2Bub and CTCF

    Jafar Sharif1, Kit-Wan Ma1,2, and Haruhiko Koseki1,

  • 1 Developmental Genetics Laboratory, RIKEN IMS, 1-7-22 Suehiro-cho, Tsurumi, Yokohama, Japan
  • 2 Current affiliation: 10X Genomics, Marunouchi 2-1-1, Chiyoda, Tokyo

P-4

Interplay between cohesin and DNA replication studied by biochemical reconstitution

    Yumiko Kurokawa1, 2, Yasuto Murayama1, 2

  • 1 National Institute of Genetics, 1111 Yata, Mishima, Shizuoka 411-8540, Japan
  • 2 Department of Genetics, SOKENDAI, 1111 Yata, Mishima, Shizuoka 411-8540, Japan
    •

P-5

How loop extrusion affects the flow properties of dense DNA

    Filippo Conforto1, Yair Augusto Gutierrez Fosado1, and Davide Michieletto1,2

  • 1 School of Physics and Astronomy, University of Edinburgh, Peter Guthrie Tait Road, Edinburgh, EH9 3FD, UK
  • 2 MRC Human Genetics Unit, Institute of Genetics and Cancer, University of Edinburgh, Edinburgh EH4 2XU, UK
    •

P-6

Embrace your DNA: What if you could hold, pull & look at single SMC complexes in action?

    Emma Verver, Frank Wang, Aida Llauro-Portell, Josephine Yeh, Andrea Candelli

    LUMICKS, Amsterdam, The Netherlands

P-7

A two-step DNA capture mediates bridging activity by single cohesin complexes

    Gemma L. M. Fisher1, Menelaos Voulgaris1, Stephen J. Cross2, Anno I Koetje1, Alice J. Francis1,3, David S. Rueda4,5 and Luis Aragon

  • 1 DNA Motors Group, MRC Laboratory of Medical Sciences, London W12 0HS, UK
  • 2 Wolfson Bioimaging Facility, University of Bristol, Bristol BS8 1TD, UK
  • 3 Cardiac Function Group, National Heart and Lung Institute, Faculty of Medicine, Imperial College London, London SW3 6LY, UK
  • 4 Single Molecule Imaging Group, MRC Laboratory of Medical Sciences, London W12 0HS, UK
  • 5 Department of Infectious Disease, Faculty of Medicine, Imperial College London, London W12 0HS, UK
    •

P-8

Phospho-regulation of condensin I: assembly and disassembly of mitotic chromatids in vitro

    Keishi Shintomi1, Yuki Masahara-Negishi1, Shoji Tane1 and Tatsuya Hirano1

  • 1 Chromosome Dynamics Laboratory, RIKEN Cluster for Pioneering Research, Wako, Saitama, Japan

P-9

Mycobacterial condensins mediate transactions of chromosomal DNA during the cell cycle

    Hanna Bułacz, Joanna Hołówka, Wiktoria Wójcik, Jolanta Zakrzewska-Czerwińska

    Department of Molecular Microbiology, University of Wrocław, Poland

P-10

Tethering NDC80-NUF2 to microparticles is sufficient to enable their biorientation in the spindle

    Kohei Asai1, Yuanzhuo Zhou1, Osamu Takenouchi1 and Tomoya S. Kitajima1,2

  • 1 Laboratory for Chromosome Segregation, RIKEN Center for Biosystems Dynamics Research (BDR), Kobe, Japan
  • 2 Graduate School of Biostudies, Kyoto University, Kyoto, Japan

P-11

Reorganization of DNA loops driven by competition between condensin I and a linker histone

    Tetsuya Yamamoto1, 2, Keishi Shintomi2, and Tatsuya Hirano2,

  • 1 Institute for Chemical Reaction Design and Discovery, Hokkaido University, Sapporo, Japan
  • 2 Chromosome Dynamics Laboratory, RIKEN, Wako, Saitama, Japan

P-12

Loop-extruding Smc5/6 organizes transcription-induced positive DNA supercoils

    Kristian Jeppsson1,2, Biswajit Pradhan3, Takashi Sutani1, Toyonori Sakata1, 2, Miki Umeda Igarashi2, Davide Giorgio Berta2, Takaharu Kanno2, Ryuichiro Nakato1, Katsuhiko Shirahige1, 2, Eugene Kim3, and Camilla Björkegren2

  • 1 Institute for Quantitative Biosciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032, Japan
  • 2 Karolinska Institutet, Department of Cell and Molecular Biology, Biomedicum, Tomtebodavagen 16, 171 77 Stockholm, Sweden
  • 3 Max Planck Institute of Biophysics, 60438 Frankfurt am Main, German

P-13

Mechanisms of Smc5/6 association of DNA junctions

    Jeremy Chang1,2 , Victoria Miller-Browne3,4, Shibai Li4, Shixin Liu1, Xiaolan Zhao4

  • 1 Laboratory of Nanoscale Biophysics and Biochemistry, The Rockefeller University, New York, NY 10065, USA
  • 2 Tri-Institutional MD-PhD Program, The Rockefeller University, Weill Cornell Medical College
  • 3 BCMB Allied Program, Weill Cornell Medicine, New York, NY, USA, 10021, USA
  • 4 Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA
    •

P-14

Centromeric cohesive cohesin mediates monoorientation in meiosis I and is lost before meiosis II

    Lucia F. Massari1, Lori B. Koch1 and Adéle L. Marston1

  • 1 Institute for Cell Biology, University of Edinburgh, Edinburgh, UK

P-15

Condensin and Telomere Segregation during Mitosis in Yeast

    C. Perini1, A. Deshayes1, C. Beneut1, K. Dubrana1, S. Mattarocci1, S. Marcand1

  • 1 Institut François Jacob, U1274 Inserm, IRCM, CEA, Université Paris-Saclay, Fontenay-aux-Roses, France
    •

P-16

Conserved Scc4-patch domain mediated cohesion establishment mechanisms in meiosis

    Dilara Kocakaplan1, Vasso Makrantoni1, Christos Spanos1, Daniel Robertson1, Juri Rappsilber1, Adele L. Marston1

  • 1 Institute for Cell Biology, University of Edinburgh, UK
    •

P-17

A Tug of War: a two-state model of Smc5/6 function regulation

    Jian Zheng1,2, Shibai Li1, Yu You3, Dinshaw Patel3, and Xiaolan Zhao1

  • 1 Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, New York, NY, 10065, USA
  • 2 Programs in Biochemistry, Cell, and Molecular Biology, Weill Cornell Graduate School of Medical Sciences, New York, NY 10065, USA
  • 3 Structural Biology Program, Memorial Sloan-Kettering Cancer Center, New York, NY, 10065, USA

P-18

How do condensin-condensin interactions contribute to mitotic chromosome assembly?

    Kazuhisa Kinoshita, Yuuki Aizawa, and Tatsuya Hirano

    Chromosnome Dynamics Laboratory, RIKEN Cluster for Pioneering Research, 2-1, Hirosawa, Wako, Saitama, Japan

P-19

Functional collaboration of condensin II and cohesin

in interphase chromosome organization

    Takao Ono1, Masatoshi Takagi2, Hideyuki Tanabe3, Tomoko Fujita4, Noriko Saitoh4, and Tatsuya Hirano1

  • 1 Chromosome Dynamics Laboratory, RIKEN, Saitama, Japan
  • 2 Cellular Dynamics Laboratory, RIKEN, Saitama, Japan
  • 3 Research Center for Integrative Evolutionary Science, The Graduate University for Advanced Studies, SOKENDAI, Kanagawa, Japan
  • 4 Division of Cancer Biology, The Cancer Institute of JFCR, Tokyo, Japan

P-20

The epigenetic reader Phf2 is positioned in the genome by cohesin-mediated DNA loop extrusion

    Wen Tang1,†, Lorenzo Costantino1,†, Roman Stocsits1, Gordana Wutz1, Ines Steinmacher1,2, Otto Hudecz1,2, Karl Mechtler1,2, Jan-Michael Peters1,*

  • 1 Research Institute of Molecular Pathology (IMP), Vienna Biocenter (VBC), Campus-Vienna-Biocenter 1, 1030 Vienna, Austria
  • 2 Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna Biocenter (VBC), 1030 Vienna, Austria
    •

P-21

Investigating the Role of Intrinsically Disordered Regions for the Regulation of Condensin Activity

    Markus Hassler1, Disha Bangalore1, Cornelia Schmidt1, Jenny Ormanns1 and Christian Haering1

  • 1 Department of Biochemistry and Cell Biology, Julius Maximilian University of Würzburg, 97074 Würzburg, Germany
    •

P-22

Progressive chromosome shape changes during cell divisions recapitulated by loop capture simulations

    Yasutaka Kakui1,2, Yoshiharu Kusano3, Maya Lopez4,5, Toru Hirota3, Bhavin S. Khatri4,5 and Frank Uhlmann4

  • 1Waseda Institute for Advanced Study, Waseda University, Tokyo 169-0051, Japan
  • 2Laboratory of Cytoskeletal Logistics, Center for Advanced Biomedical Sciences, Waseda University, Tokyo 162-8480, Japan
  • 3Division of Experimental Pathology, Cancer Institute of the Japanese Foundation for Cancer Research, Tokyo 135-8550, Japan
  • 4Chromosome Segregation Laboratory, The Francis Crick Institute, London NW1 1AT, UK
  • 5Department of Life Sciences, Imperial College London, Silwood Park Campus, Ascot SL5 7PY, UK

P-23

The analysis of the role and function of ANKRD11, a causative gene of CdLS

    DENG HUIZI1, Masashige Bando1, Shoin Tei1, Takashi Sutani1 and Katsuhiko Shirahige1

  • 1 Laboratory of Genome Structure and Function, Institute for Quantitative Biosciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, Japan
    •

P-24

Characterization of the C. elegans condensin IDC complex and its role in dosage compensation

    Antonio Valdes1, Gurumoorthy Amudhan1, Brigitta Wilde1, Peter Meister2 and Christian Haering1

  • 1 Chromosome structure and Dynamics; Julius-Maximilians-Universitat Wuerzburg (JMU); Wuerzburg; 97074; Germany
  • 2 Cell Fate and Nuclear Organization; University of Bern; Bern; CH-3012; Switzerland
    •

P-25

Two CTCF motifs impede cohesion-mediated DNA loop extrusion

    Roman Barth1†, Richard Janissen1†, Laura Muras1†, Jaco van der Torre1, Gabriele Litos2, Eli van der Sluis1, Ashmiani van den Berg1, Iain F. Davidson2, Jan-Michael Peters2, and Cees Dekker1

  • 1 Department of Bionanoscience, Kavli Institute of Nanoscience Delft, Delft University of Technology, Delft, The Netherlands
  • 2 Research Institute of Molecular Pathology, Vienna BioCenter, Vienna, Austria
    • †. These authors contributed equally to this work.

P-26

Role of DNA catenation in sister chromatid cohesion

    Aditi Kaushik1, and Madhusudhan Srinivasan1,

  • 1 DNA topology Lab, Department of Biochemistry, South Parks Road, University of Oxford, Oxford, OX1 3QU, United Kingdom
    •

P-27

Investigating models for sister chromatid cohesion establishment: a look inside budding yeast

    Kimberly Quililan1 and Frank Uhlmann1

  • 1 Chromosome Segregation Laboratory , The Francis Crick Institute, London, United Kingdom
    •

P-28

The consequences of disturbed chromosome organisation in Streptomyces venezuelae

    Pawlikiewicz Katarzyna1, Strzałka Agnieszka1, Jakimowicz Dagmara1

  • 1 Department of Molecular Microbiology, Faculty of Biotechnology, University of Wroclaw, Poland
    •

P-29

Controlling mechanisms of the Scc2-cohesin interaction to restrict peri-centromeric DNA loop expansion and facilitate mitotic chromosome segregation

    Anh Sao Nguyen1, Toyonori Sakata1,2,3, Katsuhiko Shirahige1,2,3*, Takashi Sutani1,4*

  • 1 Institute for Quantitative Biosciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-0032, Japan
  • 2 Department of Cell and Molecular Biology, Karolinska Institutet, Sweden, 171 77, Stockholm, Sweden
  • 3 Department of Biosciences and Nutrition, Karolinska Institutet, Sweden, 171 77, Stockholm, Sweden
    •

P-30

Single molecule analysis of the interplay between Human cohesin and TOP2α at DNA catenation sites

    Sanjana Saravanan1, Erin E. Cutts1,2, David Rueda1,3 and Luis Aragón1

  • 1 MRC Laboratory of Medical Sciences, Du Cane Road, London, W12 0NN, UK
  • 2 School of Biosciences, University of Sheffield, Firth Court, Sheffield, S10 2TN, UK
  • 3 Department of Infectious Disease, Faculty of Medicine, Imperial College London, Du Cane Road, London W12 0HS, UK
    •

P-31

CdLS and CHOPS syndrome are commonly characterized by cohesin loss and accumulation of the super elongation complex

    Toyonori Sakata1,2,3, Shoin Tei1, Kosuke Izumi4,5,6,7, Ian D. Krantz4,5, Masashige Bando1 and Katsuhiko Shirahige1,2,3

  • 1 Laboratory of Genome Structure and Function, Institute for Quantitative Biosciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-Ku, Tokyo 113-0032, Japan
  • 2 Karolinska Institutet, Department of Medicine, Huddinge, Biosciences and Nutrition Unit, Neo, Hälsovägen 7c, 141 83 Huddinge, Sweden
  • 3 Karolinska Institutet, Department of Cell and Molecular Biology, Biomedicum, Tomtebodavägen 16, 171 77 Stockholm, Sweden
  • 4 Division of Human Genetics, Department of Pediatrics, The Children's Hospital of Philadelphia, 3401 Civic Center Boulevard, Philadelphia, PA 19104, USA
  • 5 Department of Pediatrics, Perelman School of Medicine, University of Pennsylvania, 3401 Civic Center Boulevard, Philadelphia, PA 19104, USA
  • 6 Laboratory of Rare Disease Research, Institute for Quantitative Biosciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-Ku, Tokyo 113-0032, Japan
  • 7 Division of Genetics and Metabolism, Department of Pediatrics, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX 75390, USA
    •

P-32

How does Wapl restrict cohesin-dependent DNA looping?

    Henri MBOUMBA1, 2, Christophe CHAPARD1,2, Agnès THIERRY3,

    Jacques SERISAY3, Romain KOSZUL3, Frederic BECKOUET1,2
  • 1 Molecular biology and cellular development unit (MCD), Toulouse, France
  • 2 Université Toulouse 3, Paul Sabatier, Toulouse, France
  • 3Pasteur institute, Paris, France
    •

P-33

SMCHD1 compacts DNA in an ATP-independent manner

    Joel Ng1, Jie Yan2,3,4, and Shifeng Xue1

  • 1 Department of Biological Sciences, National University of Singapore, Singapore
  • 2 Department of Physics, National University of Singapore, Singapore
  • 3 Centre for Bioimaging Sciences, National University of Singapore, Singapore
  • 4 Mechanobiology Institute, National University of Singapore, Singapore
    •

P-34

A new structure of Cohesin in a cohesive state?

    Maurici Brunet Roig1, Byung-Gil Lee2, Madhusudhan Srinivasan1, Jan Lowe2 and Kim A Nasmyth1

  • 1 Department of Biochemistry, University of Oxford, United Kingdom
  • 2 MRC Laboratory of Molecular Biology, Cambridge, United Kingdom
    •

P-35

Scc3, Pds5, and Wapl promote in vitro DNA entrapment through binding the N-terminus of Scc1

    James Collier1, 2 and Kim Nasmyth1,

  • 1 Department of Biochemistry, University of Oxford, Oxford, UK
  • 2 Laboratory of Molecular Biology, Cambridge, UK
    •

P-36

Studying the deleterious interaction of SMC5/6 with the Epstein-Barr virus factor BNRF1

    Richard Bartlett1, Erin Cutts1,2, Ricardo Aramayo1, Nora Cronin3 and Luis Aragón1

  • 1 MRC Laboratory of Medical Sciences, Du Cane Road, London, W12 0NN, UK
  • 2 School of Biosciences, University of Sheffield, Firth Court, Sheffield, S10 2TN, UK
  • 3 Francis Crick Institute, 1 Midland Rd, London, NW1 1AT, UK
    •

P-37

Exploring the Molecular Factors Influencing Cohesin Retention at Transcription Sites

    Laura Chaptal1, Antoine Barthe1, Alba Torán Vilarrubias1, Alexandros Minakakis2, Domenico Libri2, Philippe Pasero1, Armelle Lengronne1

  • 1 Institute of Human Genetics, CNRS, UMR9002, Montpellier, France
  • 2 Institute of Molecular Genetics of Montpellier, CNRS, UMR5535, Montpellier, France

P-38

Transcription factors and flowering genes in the context of chromatin loops in cherry blossom

    Andrea Ghelfi1, Sachiko Isobe2, and Kenta Shirasawa3

  • 1 Bioinformation and DDBJ Center, National Institute of Genetics, 1111, Yata, Mishima, Shizuoka, Japan
  • 2 Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1, Yayoi, Bunkyo-ku, Tokyo, Japan
  • 3 Kazusa DNA Research Institute, 2-chōme-6-7 Kazusakamatari, Kisarazu, Chiba, Japan

P-39

Cohesin can topologically bind to ssDNA in the absence of ATP and the loader proteins

    Katsunori Fujiki1, Katsuhiko Shirahige1

  • 1 Laboratory of Genome Structure and Function, Institute for Quantitative Biosciences, The University of Tokyo, Tokyo, Japan
    •

P-40

AT-hook in STAG1 is a critical element for efficient DNA loop extrusion by cohesin

    Ryota Sakata1, Ana Losada2, and Tomoko Nishiyama1,

  • 1 Genome integrity and control laboratory, Department of Biophysics, Division of Biological Sciences, Kyoto University, Sakyo-ku Kyoto, Japan
  • 2 Chromosome Dynamics Group, Molecular Oncology Programme, Spanish National Cancer Research Centre (CNIO), Madrid, Spain
    •

P-41

Higher-order structure of chromatin regulates chromatin stiffness

    Shiori Iida1, 2, Masahito Tanaka3, Sachiko Tamura1, Yuta Shimamoto2,3, and Kazuhiro Maeshima1,2

  • 1 Genome Dynamics Laboratory, National Institute of Genetics, 1111, Yata, Mishima, Shizuoka, Japan
  • 2 The Graduate University for Advanced Studies, SOKENDAI, 1111, Yata, Mishima, Shizuoka, Japan
  • 3 Phisics and Cell Biology Laboratory, National Institute of Genetics, 1111, Yata, Mishima, Shizuoka, Japan
    •

P-42

Cohesin stabilizes RNA Pol II pausing and transcription elongation

    Shoin Tei1, Masashige Bando1, Katsuhiko Shirahige1,2,3

  • 1 1Laboratory of Genome Structure and Function, Institute for Quantitative Biosciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-Ku, Tokyo, 113-0032, Japan
  • 2 Karolinska Institutet, Department of Biosciences and Nutrition, Biomedicum, Quarter A6, 171 77, Stockholm, Sweden
  • 3 Karolinska Institutet, Department of Cell and Molecular Biology, Biomedicum, Quarter A6, 171 77, Stockholm, Sweden
    •

P-43

Novel Insights into Smc5/6 activity on single-stranded DNA

    Anno Koetje1, Erin Cutts1, Gemma Fisher1, Luis Aragon1,

  • 1 MRC Laboratory of Medical Sciences & Imperial College London Faculty of Natural Sciences & Faculty of Medicine, London
    •

P-44

Transcription promotes long range chromatin loops and acts as a barrier but not as molecular motor for cohesin-dependant loop expansion

    Christophe Chapard1,Ψ,#,Nathalie Bastié2,Ψ,&, Axel Cournac1,Ψ, Laura Chaptal3 Ψ, Henri Mboumba2, Agnes Thierry1, Olivier Gadal2, Armelle Lengronne3, Romain Koszul1*, Frédéric Beckouët2*

  • 1 Institut Pasteur, CNRS UMR 3525, Université Paris Cité, Unité Régulation Spatiale des Génomes, 75015 Paris, France
  • 2 Molecular, Cellular and Developmental biology department (MCD), Centre de Biologie Intégrative (CBI), Université de Toulouse, CNRS, UPS, 31062, Toulouse, France
  • 3 Institut de Génétique Humaine Université de Montpellier, CNRS, Equipe labélisée Ligue contre le Cancer Montpellier France
    • # present address: Molecular, Cellular and Developmental biology department (MCD), Centre de Biologie Intégrative (CBI), Université de Toulouse, CNRS, UPS, 31062, Toulouse, France & present address: Lymphocyte Development Group, MRC London Institute of Medical Sciences, Imperial College London, Hammersmith Hospital Campus, Du Cane Road, London, W12 0NN, UK Ψ These authors contributed equally * Corresponding authors: romain.koszul@pasteur.fr, frederic.beckouet@univ-tlse3.fr

P-45

Imaging of cohesion conformational dynamics using HS-AFM and MINFLUX imaging

    Sabrina M. Horn1,2, Alba Gómez-Segalàs1,2, Benedikt Bauer1, Francisco Balzarotti1 and Jan-Michael Peters1

  • 1 Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC) Campus-Vienna-Biocenter 1, 1030 Vienna, Austria
  • 2 Vienna BioCenter PhD Program, Doctoral School of the University of Vienna and Medical University of Vienna, A-1030 Vienna, Austria
    •

P-46

Cohesin supercoils DNA during loop extrusion

    Iain F. Davidson1, Roman Barth2, Sabrina Horn1,3, Richard Janissen2, Kota Nagasaka1, Wen Tang1, Gordana Wutz1, Roman Stocsits1, Benedikt Bauer1,4, Cees Dekker2 and Jan-Michael Peters1

  • 1 Research Institute of Molecular Pathology; Vienna BioCenter, Vienna, Austria
  • 2 Department of Bionanoscience, Kavli Institute of Nanoscience Delft, Delft University of Technology; Delft, The Netherlands
  • 3 Vienna BioCenter PhD Program, Doctoral School of the University of Vienna and Medical University of Vienna; Vienna, Austria
  • 4 Present address: BioNTech R&D Austria, Vienna
    •

P-47

Genome wide characterization of the chromatin localization of the human SMC5/6 complex

    Eric Maurer1, 2, Roman Stocsits1, Wen Tang1, Hiromi Tagoh1, Jan-Michael Peters1

  • 1 Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), Campus-Vienna-Biocenter 1, 1030 Vienna, Austria
  • 2 Vienna BioCenter PhD Program, Doctoral School of the University of Vienna and Medical University of Vienna, A-1030, Vienna, Austria
    •

P-48

Studying the conformational states of cohesin using cryo-EM.

    Mariia Popova1,2, Sascha J. Amann1,2, Benedikt Bauer1, David Haselbach1 and Jan-Michael Peters1

  • 1 Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), Campus-Vienna-Biocenter 1, 1030 Vienna, Austria
  • 2 Vienna BioCenter PhD Program, Doctoral School of the University of Vienna and Medical University of Vienna, A-1030, Vienna, Austria
    •

P-49

RAD21 cohesin loaded during the pre-meiotic S phase is dispensable for the regulation of male meiosis.

    Hironori Abe1, Masato Kanemaki2, Kei-ichro Ishiguro1,

  • 1 Department of Chromosome Biology, Institute of Molecular Embryology and Genetics (IMEG), Kumamoto University, Honjo 2-2-1, Chuo-ku, Kumamoto, Japan
  • 2 Department of Chromosome Science, National Institute of Genetics, 1111, Yata, Mishima, Shizuoka, Japan
    •

P-50

How PDS5 proteins control cohesin-mediated DNA loop extrusion

    Gordana Wutz1, Ryotaro Kawasumi2,4, Roman Stocsits1, Wen Tang1, Kota Nagasaka1, Iain Davidson1, Ralf Jansen5, Dana Branzei2,3 and Jan-Michael Peters1

  • 1 Research Institute of Molecular Pathology (IMP), Vienna Biocenter (VBC), Campus-Vienna-Biocenter 1, 1030 Vienna, Austria
  • 2 IFOM-the FIRC Institute of Molecular Oncology, Via Adamello 16, 20139 Milan, Italy
  • 3 Institute of Molecular Genetics, Pavia, Italy
  • 4 Present address: Department of Chemistry, Graduate School of Science,Tokyo Metropolitan University
  • 1-1 Minami-Osawa, Hachioji, Tokyo 192-0397, Japan
  • 5 IMBA - Institute of Molecular Biotechnology, Dr.-Bohr-Gasse 3,1030 Vienna, Austria

P-51

Reconstituted long chromatin array reveals modification pattern-dependent structure

    Yohsuke T. Fukai1, Tomoya Kujirai2, Masatoshi Wakamori3, Hitoshi Kurumizaka2, Takashi Umehara3, Kyogo Kawaguchi1,4,5

  • 1 Nonequilibrium Physics of Living Matter RIKEN Hakubi Research Team, RIKEN Center for Biosystems Dynamics Research, 2-2-3 Minatojima-minamimachi, Chuo-ku, Kobe 650-0047, Japan
  • 2 Laboratory of Chromatin Structure and Function, Institute for Quantitative Biosciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032, Japan
  • 3 Laboratory for Epigenetics Drug Discovery, RIKEN Center for Biosystems Dynamics Research, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan
  • 4 RIKEN Cluster for Pioneering Research, 2-2-3 Minatojima-minamimachi, Chuo-ku, Kobe 650-0047, Japan
  • 5 Institute for Physics of Intelligence, The University of Tokyo, 7-3-1 Hongo, Tokyo 113-0033, Japan
    •

P-52

HP1-binding protein PRR14 interacts with condensin II and contributes to the progression of chromosome formation during mitosis

    Aoi Takami, Suzuha Gose, Yuto Imai, Ryota Mochizuki, Shin-Ya Isobe and Chikashi Obuse

    Department of Biological Sciences, Graduate School of Science, Osaka University

P-53

Plasmid recognition and cleavage by DNA-measuring SMC JET nucleases

    Florian Roisné-Hamelin1,3, Hon Wing Liu1,3, Michael Taschner1, Yan Li1, Bertrand Beckert2, Alexander Myasnikov2, Stephan Gruber1,4

  • 1 Department of Fundamental Microbiology (DMF), Faculty of Biology and Medicine (FBM), University of Lausanne (UNIL), 1015 Lausanne, Switzerland
  • 2 Dubochet Center for Imaging (DCI), EPFL, 1015 Lausanne, Switzerland
  • 3 These authors contributed equally to this work
  • 4 Corresponding author: stephan.gruber@unil.ch

P-54

Co-evolution of bacteria RecA-RecN interaction interfaces

    Mizuki Inoue1, Genki Akanuma2, Shunsuke Noda3, Masafumi Hayashi1 and Takashi Hishida1

  • 1 Graduate School of Life Science, Gakushuin University, 1-5-1, Mejiro, Toshima-ku, Tokyo, Japan
  • 2 Faculty of Chemical Science, Josai University, Sakado, Saitama, Japan
  • 3 Laboratory, National Institute of Genetics, 1111, Yata, Mishima, Shizuoka, Japan
    •

P-55

PCNA recruits NIPBL to promote sister chromatid cohesion

    Ryotaro Kawasumi1, 2, Ivan Psakhye2, Takuya Abe1, Kouji Hirota1 and Dana Branzei2

  • 1 Department of Chemistry, Graduate School of Science, Tokyo Metropolitan University, Minamiosawa 1-1, Hachioji-shi, Tokyo, Japan
  • 2 IFOM ETS, the AIRC Institute of Molecular Oncology, Milan, Italy
    •

P-56

Single particle tracking to understand the dynamics of cohesin throughout the cell cycle

    Hazel Wake1 and Madhusudhan Srinivasan1

  • 1 Department of Biochemistry , University of Oxford, South Parks Road, Oxford OX1 3QU, UK
    •

P-57

Characterize context-specific protein interactions by integrating gene expression and essentiality

    Gina M Oba1 and Ryuichiro Nakato1

  • 1 Laboratory of Computational Genomics , Institute for Quantitative Biosciences, The University of Tokyo, Yayoi, Bunkyo-ku, Tokyo, Japan

P-58

Interaction between PP2A and condensing II regulates mitotic chromosome dynamics

    Kazashi Kato1, 2, Sae Higashiura1, Shunsuke Arimoto1 and Keiji Kimura1, 2

  • 1 Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tenno-dai, Tsukuba Science City, Ibaraki, Japan
  • 2 TARA center, University of Tsukuba, 1-1-1 Tenno-dai, Tsukuba Science City, Ibaraki, Japan
    •

P-59

An SMC-dependent role of TFIIIC in genome organization

    Daniel Obaji1, Jun Kim1, Yetunde Olagbegi1, Anh-Thu Le1 and Sevinc Ercan1

  • 1 Department of Biology, Center for Genomics and Systems Biology, New York University, New York, NY, USA
    •

P-60

HDAC8 differentially deacetylates cohesinSA1 and cohesinSA2

    Marjon S. van Ruiten1, Tajith B. Shaik2, Klaudia Majszczyk1, Clément Rouillon1, Astrid Fauster3, Ren Xie3, Thomas Klompstra1, Démi van Gent1, Katsuhiko Shirahige4, Anastassis Perrakis3, Thijn R. Brummelkamp3, Daniel Panne2, and Benjamin D. Rowland1

  • 1 Division of Cell Biology, The Netherlands Cancer Institute, Amsterdam, 1066 CX, The Netherlands
  • 2 Leicester Institute of Structural and Chemical Biology, Department of Molecular and Cell Biology, University of Leicester, Leicester, UK
  • 3 Division of Biochemistry, Oncode Institute, The Netherlands Cancer Institute, Amsterdam, 1066 CX, The Netherlands
  • 4 Institute for Quantitative Bioscience, Tokyo University, Tokyo, 113-0032, Japan
    •

P-61

Shaping the genome: Condensin II activation by M18BP1

    Rebecca J. Harris1, Tom F. Aarts1, Ren Xie2,3, Abdelghani Mazouzi2,3, Sushweta Sen1, Claire Hoencamp1, Judith H.I. Haarhuis1, Anastassis Perrakis2,3, Thijn R. Brummelkamp2,3 and Benjamin D. Rowland1

  • 1 Division of Cell Biology, The Netherlands Cancer Institute, Amsterdam, Netherlands
  • 2 Oncode Institute, Utrecht, Netherlands
  • 3 Division of Biochemistry, The Netherlands Cancer Institute, Amsterdam, Netherlands
    •

P-62

A two-step mechanism shaping the genome-wide pattern of sister chromatid cohesion

    Takashi Sutani, Mai Ishibashi, Atsunori Yoshimura, Masashige Bando, Katsuhiko Shirahige

    Institute for Quantitative Biosciences, The University of Tokyo. Bunkyo-ku, Tokyo, Japan

P-63

Functional analysis of intragenic cohesin binding sites negatively correlated with transcriptional regulation

    Yuya Nagaoka1 and Ryuichiro Nakato1,

  • 1 Laboratory of Computational Genomics, Institute for Quantitative Biosciences, The University of Tokyo, Bunkyo-ku, Tokyo, 113-0032, Japan
    •

P-64

Transcriptional regulation mechanism involving cohesin loader NIPBL/MAU2

    Atsunori Yoshimura1, Rei Kajitani2, Shoin Tei1, Toyonori Sakata1, Takashi Sutani1, Takehiko Itoh2, Masashige Bando1, Katsuhiko Shirahige1,

  • 1 IQB, The University of Tokyo, Tokyo, Japan
  • 2 School of Life Science and Technology, Tokyo Institute of Technology, Tokyo, Japan
    •

P-65

Genome instability and senescence are markers of Cornelia de Lange syndrome cells

    Maddalena Di Nardo1, Ian D. Krantz2,3 and Antonio Musio1

  • 1 Institute for Biomedical Technologies, National Research Council, Pisa, Italy
  • 2 Roberts Individualized Medical Genetics Center, Division of Human Genetics, The Children’s Hospital of Philadelphia, Philadelphia, USA
  • 3 Perelman School of Medicine, The University of Pennsylvania, Philadelphia, USA
    •

P-66

Mechanistic insights into non-SMC subunits mediated DNA loading in cohesin complexes

    Yoshimi Kinoshita, Tomoko Nishiyama

    Division of Biology, Graduate School of Science, Kyoto University, Kyoto, Japan

P-67

PARP1 levels and remaining cohesin functionality are determinants of PARPi sensitivity in STAG2 deficient cancer cells

    Thom Molenaar1, Zoi Karagiorgou1, Daan Tang1, Sacha Jacobs1, Isha Sadal1, Martin Rooimans1, Job de Lange1

  • 1 Amsterdam UMC, department of Human Genetics, Amsterdam, The Netherlands

P-68

Role of Topoisomerase II and Cohesin in sister chromatid cohesion and segregation

    Minakshi Sharma1, and Madhusudhan Srinivasan1,

  • 1 Department of Biochemistry, University of Oxford, South Parks Rd, OX1 3QU Oxford, Oxfordshire, England

P-69

How Condensin I and KIF4A drive mitotic chromosome assembly in mitosis

    Motoko Takahashi1, Chang Liu1 and Toru Hirota1

  • 1 Division of Experimental Pathology, The Cancer Institute, Japanese Foundation for Cancer Research (JFCR), 3-8-31, Ariake, Koto-ku, Tokyo, Japan
    •

P-70

Telomeres stall DNA loop extrusion by condensin

    Brian T. Analikwu1*, Alice Deshayes2*, Jaco van der Torre1, Thomas M. Guérin3, Allard J. Katan1, Claire Béneut2, Roman Barth1, Jamie Phipps2, Vittore Scolari4, Xavier Veaute2, Christopher Barrington5, Didier Busso2, Frank Uhlmann3, Karine Dubrana2, Stefano Mattarocci2$, Cees Dekker1$, Stéphane Marcand2$

  • 1 Kavli Institute of Nanoscience Delft, Delft University of Technology, Delft, the Netherlands
  • 2 Université Paris-Saclay, Université Paris-Cité, CEA, Inserm, Institut de biologie François Jacob, UMR Stabilité Génétique Cellules Souches et Radiations, Fontenay-aux-Roses, France
  • 3 Chromosome Segregation Laboratory, The Francis Crick Institute, London, United Kingdom
  • 4 Institut Curie, PSL Research University, Sorbonne Université, Paris, France
  • 5 Bioinformatics and Biostatistics Science Technology Platform, The Francis Crick Institute, London, United Kingdom
    • * Shared first author with equal contribution $ Corresponding authors: stefano.mattarocci@inserm.fr, c.dekker@tudelft.nl, stephane.marcand@cea.fr

P-71

Symmetry in Loop Extrusion by Dimeric and Monomeric SMC Complexes

    Biswajit Pradhan1, Takaharu Kanno2, Adrian Pinto3, Martin D. Baaske1, Damla Tetiker1, Erin Cutt4, Constantinos Chatzicharlampous5, Herwig Schüler5, Amar Deep6, Kevin D. Corbett6, Luis Aragon4, Peter Virnau3, Camilla Björkegren2, Eugene Kim1

  • 1 Max Planck Institute of Biophysics, 60438 Frankfurt am Main, Germany
  • 2 Karolinska Institutet, Department of Cell and Molecular Biology; Biomedicum, Tomtebodavägen 16, 171 77, Stockholm, Sweden
  • 3Institut für Physik, Johannes Gutenberg-Universität Mainz, DE-55122 Mainz, Germany
  • 4DNA Motors Group, MRC Laboratory of Medical Sciences (LMS), Du Cane Road, London W12 0HS, UK
  • 5Center for Molecular Protein Science, Department of Chemistry, Lund University, 22100 Lund, Sweden
  • 6Department of Molecular Biology, University of California San Diego, La Jolla CA 92093 USA
    •

P-72

SMC and HupS proteins cooperate in global chromosome rearrangements during sporogenic differentiation of Streptomyces venezuelae

    Julia Duława-Kobeluszczyk1, Tomasz Małecki1, Agnieszka Strzałka1, Tomasz Małecki1, Katarzyna Pawlikiewicz1, Michał Tracz1, Dagmara Jakimowicz1, Marcin Szafran1

  • 1 Faculty of Biotechnology, University of Wrocław, Joliot-Curie 14A, 50-383 Wrocław, POLAND
    •

P-73

An Acetylation Complex Controls Sister Chromatid Cohesion Establishment

    Céline Bouchoux and Frank Uhlmann

  • Chromosome Segregation Laboratory. The Francis Crick Institute. London, UK
    •

P-74

SMC5/6 confines and assists releasing DNA supercoils during replication

    Yoshiharu Kusano1, and Toru Hirota1

  • 1Division of Experiemntal Pathology, Cancer Institue, Japanese Foundation for Cancer Research, Kotoku Tokyo, Japan
    •

P-75

Nucleosome arrays maintained by the FACT histone chaperone during mitosis hinder chromatin folding and mitotic chromosome condensation by condensin

    Léonard Colin, Esther Toselli, Ramachandran Boopathi and Pascal Bernard

    Laboratory of Biology and Modelling of the Cell. CNRS & Ecole Normale Supérieure de Lyon, UMR5239, Lyon, France

P-76

NIPBL disruption in Cornelia de Lange Syndrome alters cohesin/NIPBL distribution, transcriptome, proteome, and genome organization

    Fernando Martinez Montañez1, Patricia Garcia1, Ana Latorre-Pellicer2, Beatriz Puisac2, Feliciano J. Ramos2, J Pié2, Ethel Queralt1

  • 1 Instituto de Biomedicina de Valencia (IBV-CSIC), Valencia, Spain
  • 2 Unit of Clinical Genetics and Functional Genomics, Department of Pharmacology-Physiology and Paediatrics, School of Medicine, University of Zaragoza, CIBERER-GCV02 and IIS Aragon, Zaragoza, Spain
    •

P-77

Molecular mechanisms of condensin II activity

    Alessandro Borsellini1, Duccio Conti2, Erin Cutts3, Rebecca J. Harrys4, Valentina Cecatiello1, Joanna J. Andrecka1, Benjamin D. Rowland4, Andrea Musacchio2 and Alessandro Vannini1,

  • 1 Human Technopole, Milan, Italy
  • 2 Department of Mechanistic Cell Biology, Max Planck Institute of Molecular Physiology, Dortmund, Germany
  • 3 School of Bioscience, University of Sheffield, Sheffield, United Kingdom
  • 4 Division of Cell Biology, The Netherlands Cancer Institute, Amsterdam, the Netherlands
    •

P-78

E. coli SMC-like protein RecN regulates chromosome dynamics and stimulates RecA-mediated repair of DNA double strand breaks.

    Shunsuke Noda1,2, Anju Niki1, Kenji keyamura1, Genki Akanuma1, Takashi Hishida1

  • 1 Department of Molecular Biology, Graduate School of Science, Gakushuin
    • University, 1-5-1 Mejiro, Toshima-ku, Tokyo, 171-8588 Japan
  • 2 Department of Chromosome Science, National Institute of Genetics, Mishima, Japan

P-79

Mutations on TopA or cell wall related genes suppressed the temperature sensitivity of ΔmukB cells

    Koichiro Akiyama, Hironori Niki

  • National Institute of Genetics, Mishima, Japan
    •

P-80

Substrate accessibility regulation of human TopIIα decatenation by cohesin

    Erin E. Cutts1, Sanjana Saravanan1, Gemma L. M. 1, David S. Rueda2,3 and Luis Aragon1

  • 1 DNA Motors Group, MRC Laboratory of Medical Sciences, Du Cane Road, London W12 0HS, UK
  • 2 Single Molecule Imaging Group, MRC Laboratory of Medical Sciences, Du Cane Road, London W12 0HS, UK
  • 3 Department of Infectious Disease, Faculty of Medicine, Imperial College London, Du Cane Road, London W12 0HS, UK
    •

P-81

NIPBL’s role in building cohesion

    Isobel Johns1, Madhusudhan Srinivasan1, and Kim Nasmyth1

  • 1 DNA topology Lab, Department of Biochemistry, South Parks Road, University of Oxford, Oxford, OX1 3QU, United Kingdom
    •

P-82

Cohesin forms fountains at active enhancers in C. elegans

    Bolaji N. Lüthi1,2, Jennifer I. Semple1, Anja Haemmerli1, Saurabh Thapliyal3, Kalyan Ghadage1,2, Klement Stojanovski6, Dario D’Asaro5, Moushumi Das1,2, Nick Gilbert3, Dominique A. Glauser4, Benjamin Towbin6, Daniel Jost5 and Peter Meister1

  • 1 Cell Fate and Nuclear Organization, Institute of Cell Biology, University of Bern, Switzerland
  • 2 Graduate School for Cellular and Biomedical Sciences, University of Bern, Switzerland
  • 3 MRC Human Genetics Unit, Institute of Genetics and Cancer, The University of Edinburgh
  • 4 Department of Biology, University of Fribourg, Fribourg Switzerland
  • 5 Laboratoire de Biologie et Modélisation de la Cellule, Ecole Normale Supérieure de Lyon, CNRS, UMR5239, Inserm U1293, Université Claude
    •


Confirmed Speakers

  • Ana Losada (Centro Nacional de Investigaciones Oncológicas)
  • Anjana Badrinarayanan (National Centre for Biological Sciences)
  • Anton Goloborodko (Institute of Molecular Biotechnology, Vienna Biocenter)
  • Antony W Oliver (University of Sussex)
  • Armelle Lengronne (CNRS)
  • Barbara Meyer (University of California, Berkeley)
  • Benjamin Rowland (The Netherlands Cancer Institute)
  • Camilla Björkegren (Karolinska Institute)
  • Cees Dekker (Delft University of Technology)
  • Christian Haering (University of Würzburg)
  • Debbie L C van den Berg (Erasmus MC)
  • Eugene Kim (Max Planck Institute of Biophysics)
  • Francesca Pisani (Institute of Biochemistry and Cell Biology - CNR)
  • Frank Bürmann (University of Oxford)
  • Frank Uhlmann (The Francis Crick Institute)
  • Hiroaki Kato (Kyoto University)
  • Jan-Michael Peters (Institute of Molecular Pathology)
  • Jolien van Hooff (Wageningen University & Research)
  • Katsuhiko Shirahige (The University of Tokyo)
  • Madhusudhan Srinivasan (University of Oxford)
  • Kazuhiro Maeshima (National Institute of Genetics)
  • Maxim Molodtsov (The Francis Crick Institute)
  • Romain Koszul (Institut Pasteur)
  • Sevinc Ercan (New York University)
  • Stephan Gruber (Université de Lausanne)
  • Stephen D Bell (The Ohio State University)
  • Takashi Akera (National Institutes of Health)
  • Tatsuya Hirano (Riken)
  • Tomoko Nishiyama (Kyoto University)
  • Toru Hirota (Japanese Foundation for Cancer Research)
  • William Earnshaw (University of Edinburgh)
  • Xiaolan Zhao (Memorial Sloan Kettering Cancer Center)
  • Xindan Wang (Indiana University)
 

Registration

  • Registration Deadline
  • 31 Aug. 2024
  • Abstract Submission Deadline
  • 31 Jul. 2024
    7 Aug. 2024
@ALTIMATE_BLUE_J
  • STUDENT/POSTDOCSEarly Bird \27,000 (JPY)
    until 15-Jul-2024
    Late Bird \30,000 (JPY)
  • ACADEMICEarly Bird \37,000 (JPY)
    until 15-Jul-2024
    Late Bird \45,000 (JPY)
  • INDUSTRYEarly Bird \50,000 (JPY)
    until 15-Jul-2024
    Late Bird \50,000 (JPY)

Registration:  Click the ONLINE APPLICATION tab above to begin the online registration process. All meeting participants must register and pay in full by 31 August 2024.

If you would like to submit your abstract, you must register and pay by 31 July 2024.


Oversubscribed Meetings:  Once a meeting has reached the capacity (200 registered participants), a special notation is included at the top of the meeting's webpage and closed the registered website immediately.


Abstract Submission

All attendees are encouraged to submit abstracts. After completing the online registration, you will receive a confirmed registration e-mail containing the website information for Abstract Submission.


Once the submission deadline has passed, the meeting's organizing committee reviews all abstracts, decides which will be selected for oral or poster presentations, and finalizes the meeting program.


 

Venue

Plaza Verde

1-1-4 Otemachi, Numazu City, Shizuoka Prefecture, Japan

How to get

Numazu City is conveniently located about 100 km from Tokyo and 1 hour by Shinkansen (bullet train), making it easy to access. How to reach the Plaza verde, 3-min walk from Numazu Station (JR) the north exit.

Click this link for details: Plaza Verde Access

 

Organizers

The SMC International Committee:
  • Hironori Niki (National Institute of Genetics, Japan)
  • Yasuto Murayama (National Institute of Genetics, Japan)
  • Katsuhiko Shirahige (University of Tokyo, Japan)
  • Tatsuya Hirano (Riken, Japan)
  • Tomoko Nishiyama (Kyoto University, Japan)
  • Frank Uhlmann (The Francis Crick Institute, United Kingdom)
  • Toru Hirota (Japanese Foundation for Cancer Research)
Symposium Organizers:
  • Hironori Niki (National Institute of Genetics, Japan)
  • Koichiro Akiyama (National Institute of Genetics, Japan)
  • Yasuto Murayama (National Institute of Genetics, Japan)

Contact

nigis2024@niki-lab.sakura.ne.jp