Microscopic technics
  ●　poly-L-lysine coat on slide glass
A method to coat slide glass with poly-L-lysine to stick cells to the slide glass for microscopic observation
E.coli
  ●　Genomic integration of GFP tagged protein in E.coli cells
A method to fuse fluorescent protein (GFP, YFP, CFP) to N or C terminal of a protein of interest.
The advantage of this method is that fusion proteins are expressed under the control of its native promoter.
●　DNA staining with hoechst 33342 dye
A method to stain chromosomal DNA in living E. coli cell, suited for live cell imaging.
●　Membrane staining with FM4-64 dye
A method to stain cell membrane of living E. coli cell, suited for live cell imaging.
Modified from Fishov et al., Mol. Microbiol. 1999, 32, 1166-1172.
●　DNA staining with DAPI
A method to stain chromosomal DNA in fixed E. coli cell.
●　FISH for E. coli cell
A method to analyze subcellular localization of specific cis site on chromosome in fixed E. coli cell.
●　Gene disruption on E. coli chromosome using recombination system
A method to delete a gene of interest using RED recombination system in E. coli cell.
The protocol was written by yamaichi 030501updated by yamaichi 040426 in Japanese from
Datsenko and Wanner, 2000, "One-step inactivation of chromosomal genes in E. coli K-12 using PCR products"
PNAS 97, 6640-6645.
FissionYeast
  ●　DNA neutral-neutral Electrophoresis (2D replication fork gel detection in S.pombe)
The method is based on agarose embedding protocol (Arcangioli B (1998) EMBO J 17: 4503−4510). This helps the preservation of fragile replication intermediates after de-proteinisation step.
Electroporation　（内部のみ）
           

国立遺伝学研究所
遺伝形質研究系 微生物機能研究室（仁木研究室）

〒411-8540 静岡県三島市谷田1111 ＞ 連絡先詳細および地図はこちら

Microbial Physiology Laboratory (Niki Laboratory)
Department of Gene Function and Phenomics
National Institute of Genetics

1111 Yata Mishima Shizuoka Japan P.O. 411-8540 > Contact details and Maps